Review Article
Open Access
Development and Validation of New UV- Spectroscopic Method for Water Soluble Folic Acid
Sandip S. Kshirsagar, Sachin M. Bhalekar*, Dipali K. Gopale, Shirishkumar D. Ambavade
JSPM’S Charak College of Pharmacy And Research, Gat No. 719/1&2. Wagholi, Pune-412207,
India
Sandip S. Kshirsagar et al /Int.J. Chemical Concepts. 2021,7(1), pp 49-55.
Abstract
In the present work, six simple and sensitive spectrophotometric have been developed for
the quantitative determination of folic acid. Method A is a UV spectrophotometric method in which
Folic acid was dissolved in Water and exhibited absorption maximum at 281 nm and obeyed Beer’s
law in the concentration range of 2-10μg/ml. Method B is the first order derivative spectroscopy method
adopted to eliminate spectral interference, in which derivative amplitude was measured at 281 nm with
n=1.
The proposed methods are economical, simple, sensitive and accurate for quantitative determination of
folic acid in bulk drug and pharmaceutical formulations.
Keywords
Folic acid; First order derivative; Area under curve.
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